Murashige and Skoog (MS) Basal MediumMurashige and Skoog (MS) Basal Medium is the most popular culture medium in modern plant tissue culture research. It is a complex growth medium with all of the micronutrients and macronutrients needed for plants to thrive. MS Medium is effective on an endless list of plant species. Murashige and Skoog Basal Medium should be dissolved at 4. 33 g L of distilled water and autoclave. Cytokines, Auxins, Vitamins, and Sucrose may be added as needed.

See a recommended protocol for lung staining by Boston University and Transformation of DH10Bac cells by Rockefeller University

tetrahydrofolate

thus reducing inconsistent results and variability

including popular choices like Murashige and Skoog (MS) Media

5g/L is recommended

McCown Woody Plant Basal Salt Mixture contains micronutrients and macronutrients

autoclaving

Contains Macronutrients and Micronutrients as described by Eriksson (1965) for cell suspension culture of haplopappus gracilis

A usage of 3

Murashige & Skoog (MS) Modified Vitamin Powder Mix is plant tissue culture tested

Usage at a concentration of 4

Plate the transformed bacteria to be tested and incubate